Deteksi cannabinoids pada air limbah dan air sungai

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 Milan S, Lelario S, Scrano L, Ottati C, Bufo SA, Alpendurada MdF. 2022. Detection of eight cannabinoids and one tracer in wastewater and river water by SPE-UPLC-ESI-MS/MS. Water, 14(4) : 588. https://doi.org/10.3390/w14040588.


Abstrak : Konsumsi obat-obatan terlarang memunculkan masalah sosial dan ekonomi global. Penggunaan metode-metode analitik, pengawasan, dan deteksi yang tepat terhadap residu obat-obatan ilegal yang berbeda-beda di dalam sampel air limbah dapat membantu menanggulangi masalah tersebut. Penelitian ini mendefinisikan sebuah metode untuk mengembangkan, memberikan validasi, dan mengaplikasikan secara praktis serta proses analitik yang menyeluruh untuk evaluasi enam cannabinoids alami (CBG, CBD, CBDV, CBN, THC, THCV), dua cannabioids di dalam bentuk asam (CBDA, THCA-A), dan metabolit manusia yang berhubungan dengan cannabis utama (THC-COOH). Setelah melalui tahap SPE offline enrichment, peneliti menggunakan sebuah sistem UPLC-ESI-MS/MSI untuk menentukan sejumlah produk samping. Matriks-matriks yang diamati adalah sampel dari asal yang berbeda-beda: (i) aliran air dari pusat penanganan air limbah di wilayah perkotaan Porto; dan (ii) air lingkungan dari Sungai Febros, yang merupakan anak sungai tepi kiri terakhir dari Sungai Douro. Pendekatan multi-residue digunakan dan berhasil diterapkan untuk menganalisis sampel air yang diambil di lokasi-lokasi tersebut di atas. Kuantifikasi cepat dan tepat terhadap sembilan cannabinoids yang berbeda di dalam sampel air yang berbeda terjadi di dalam sembilan menit pada level ng L^-1. Keberadaan belasan ng L^-1 dari sejumlah metabolit sekunder cannabis, seperti CBD, CBDA, CBN, THCA-A, menunjukkan adanya penggunaan besar-besaran terhadap spesies ini di lingkungan tempat penelitian dilakukan. 

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